Abstract: The toxicity of the active molecule in herbicides has been used to determine safe concentrations, because other components are considered inert. Roundup, which contains the active molecule Glyphosate, was described as an endocrine disrupter because non-cytotoxic concentrations inhibited progesterone synthesis in vitro. Human chorioplacental JAr cells synthesise progesterone, and increase synthesis when stimulated by chorionic gonadotrophin (hCG), or the transduction molecule cAMP. JAr cells were exposed to two Roundup formulations, and compared with the same concentrations of glyphosate ± cAMP, or ± hCG for 1, 4, 24, 48 or 72h. The surviving viable cells were quantified using an MTT assay, and progesterone was measured in an ELISA. hCG and cAMP stimulated progesterone synthesis by cells in vitro as expected. In contrast to previous reports, JAr cell death preceded decreased progesterone synthesis, and steroidogenesis was unaffected by low, non-cytotoxic concentrations of Roundup or glyphosate. Roundup was more cytotoxic than glyphosate alone; the 24h EC50 was 16mM for glyphosate, but 0.